Proceeds from the sale, which fetched £2.92 million ($3.18 million), will be donated to charity, along with receipts from the sale of 24 other lots auctioned at a special sale of items marking 60 years of 007.
The replica comes fitted with imitation machine guns behind the headlights.
A further 35 lots have been put up for auction online until October 5.
The replica of the iconic Aston, one of eight specially designed and manufactured for the 2021 film, is the only DB5 stunt car put up for sale by Aston Martin and EON productions, according to Christie's.
Other items on sale include cars as well as notable costumes and accessories linked to the franchise.
